Specifications
| Size | 50μL/100μL |
| Target | BDNF |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 28kD |
| MW(Observed) | Precursor 28kD,Mature 12-14kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:2000-1:10000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0858R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | BDNF |
| Protein Name | BDNF(Brain Derived Neurotrophic Factor) |
| Other Name | Brain-derived neurotrophic factor; / BDNF; / Abrineurin |
| Species | Human |
| Gene ID | |
| Uniprot | P23560, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P21237, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | P23363 |
| Cellular Localization | Secreted .; [BDNF precursor form]: Secreted . A proportion of BDNF is secreted as immature precursor (proBDNF). . |
| Tissue Expression | Detected in blood plasma and in saliva (at protein level) (PubMed:11152678, PubMed:19467646). Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta |
| Signaling_pathway | |
| Research Areas | >>MAPK signaling pathway; / >>Ras signaling pathway; / >>cAMP signaling pathway; / >>PI3K-Akt signaling pathway; / >>Neurotrophin signaling pathway; / >>Huntington disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Cocaine addiction; / >>Alcoholism |
| Function | Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed:11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability |
