Price range: $268.00 through $328.00
Background:
This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010],
| Size | 50μL/100μL |
| Target | BLK |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 58kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BLK. AA range:456-505 |
| Specificity | Blk Polyclonal Antibody detects endogenous levels of Blk protein. |
| Gene Name | BLK |
| Protein Name | Tyrosine-protein kinase Blk |
| Other Name | BLK; / Tyrosine-protein kinase Blk; / B lymphocyte kinase; / p55-Blk |
| Species | Human |
| Gene ID | 640 |
| Uniprot | P51451, |
| Species.1 | Mouse |
| Gene ID.1 | 12143 |
| Uniprot.1 | P16277 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell membrane; Lipid-anchor . Present and active in lipid rafts. Membrane location is required for the phosphorylation of CD79A and CD79B (By similarity). . |
| Tissue Expression | Expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles. |
| Signaling_pathway | Organismal Systems >> Immune system >> B cell receptor signaling pathway |
| Research Areas | |
| Function | Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Function:May function in a signal transduction pathway that is restricted to B-lymphoid cells.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain., |
