Bmi-1 Monoclonal Antibody
Ucallm_WM0057-50
Price range: $268.00 through $328.00
Product details
Background:
BMI1 proto-oncogene, polycomb ring finger(BMI1) Homo sapiens This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015],
Specifications
| Size | 50μL/100μL |
| Target | Bmi-1 |
| Reactivity | Human |
| Applications | WB, IHC, IF, FC, ELISA |
| MW(Calculated) | 37kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 12G1 |
| Immunogen | Purified recombinant fragment of human Bmi-1 expressed in E. Coli. |
| Specificity | Bmi-1 Monoclonal Antibody detects endogenous levels of Bmi-1 protein. |
| Gene Name | BMI1 |
| Protein Name | Polycomb complex protein BMI-1 |
| Other Name | BMI1; / PCGF4; / RNF51; / Polycomb complex protein BMI-1; / Polycomb group RING finger protein 4; / RING finger protein 51 |
| Species | Human |
| Gene ID | 648 |
| Uniprot | P35226, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P25916 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Cytoplasm . |
| Tissue Expression | Epithelium,Erythrocyte,Muscle,Thymus, |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer |
| Research Areas | >>Signaling pathways regulating pluripotency of stem cells; / >>Transcriptional misregulation in cancer; / >>MicroRNAs in cancer |
| Function | Disease:Cooperates with the MYC oncogene to produce B-lymphomas.,Function:Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.,PTM:May be polyubiquitinated; which does not lead to proteasomal degradation.,similarity:Contains 1 RING-type zinc finger.,subunit:Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Also component of E3 ubiquitin ligase complex or human polycomb repressive complex 1-like at least composed of PHC2, BMI1, RING1 and RNF2. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1., |
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