Name: BRG1 mouse mAb
SKU: WM0708
Price: 268 USD
Availability: InStock

Product details

Background:
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012],

Specifications

Size	50μL/100μL
Target	BRG1
Reactivity	Human, Mouse
Applications	WB, IF, IP
MW(Calculated)
MW(Observed)	220kD
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:1000; ICC 1:50; IF 1:50-200
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	18C5
Immunogen	Purified recombinant human BRG1 protein fragments expressed in E.coli.
Specificity	This antibody detects endogenous levels of BRG1 and does not cross-react with related proteins.
Gene Name	SMARCA4
Protein Name
Other Name	ATP dependent helicase SMARCA4; / ATP-dependent helicase SMARCA4; / BAF 190; / BAF190; / BAF190A; / Brahma protein homolog 1; / Brahma protein like 1; / BRG1; / BRG1 associated factor 190A; / BRG1 protein; / BRG1-associated factor 190A; / BRM/SWI2 related gene 1; / Global transcription activator homologous sequence; / global transcription activator snf2l4; / Homeotic gene regulator; / hSNF2b; / Mitotic growth and transcription activator; / MRD16; / Nuclear protein GRB1; / Protein brahma homolog 1; / Protein BRG-1; / Protein BRG1; / RTPS2; / SMARC A4; / SMARCA4; / SMCA4_HUMAN; / SNF2; / SNF2 beta; / SNF2 like 4; / SNF2-beta; / SNF2B; / SNF2L4; / SNF2LB; / Sucrose nonfermenting like 4; / SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4; / SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4; / SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; / SWI2; / Transcription activator BRG1.
Species	Human
Gene ID	6597
Uniprot	P51532,
Species.1	Mouse
Gene ID.1	20586
Uniprot.1	Q3TKT4
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Nucleus . Colocalizes with long non-coding RNA Evf2 in nuclear RNA clouds. .
Tissue Expression	Colocalizes with ZEB1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level).
Signaling_pathway	Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Genetic Information Processing >> Chromosome >> ATP-dependent chromatin remodeling
Research Areas	>>Thermogenesis; / >>Hepatocellular carcinoma
Function	Function:Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the SNF2/RAD54 helicase family.,similarity:Contains 1 bromo domain.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 1 HSA domain.,subunit:Interacts with NR3C1, PGR, SMARD1, TOPBP1 and ZMIM2/ZIMP7. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Component of the BAF53 complex, at least composed of BAF53A, RUVBL1, SMARCA4/BRG1, and TRRAP, which preferentially acetylates histone H4 (and H2A) within nucleosomes. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B.,