SKU: WM1078 Categories: ,

C. Elegans β-actin Mouse mAb [Mix-mA]

Price range: $268.00 through $328.00

SKU: WM1078-50
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Product details

Background:
β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.

Specifications

TargetActin β
ReactivityC.elegans, Human, Mouse, Rat
ApplicationsIHC, WB
MW(Calculated)
MW(Observed)42kD
Host SpeciesMouse
IsotypeIgG2a,kappa
Conjugate/ModificationUnmodified
Modification
Recommended Dilution RatioIHC1:200-400; WB 1:5000-20000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Purity
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityMonoclonal
Clone Number12H2
ImmunogenSynthesized peptide derived from C. Elegans β-actin
Sequence
SpecificityThis antibody detects endogenous levels of C. Elegans β-actin
Gene NameC. Elegans β-actin
Protein NameC. Elegans β-actin
Other NameACTB; / Actin; / cytoplasmic 1; / Beta-actin; / Actin β; / β-Actin; / Beta actin; / B actin; / actin Beta
SpeciesHuman
Gene ID-1
UniprotP0DM41
Species.1
Gene ID-2
Uniprot.1
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Backgroundβ-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.
Cellular Localization
Tissue Expression
Signaling_pathway
Research Areas
Function
RRID
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