Name: CaMKK2 (Phospho Ser511) rabbit pAb
SKU: WP10311
Price: 288 USD
Availability: InStock

Product details

Background:

The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. The major isoform of this gene plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Protein products of this gene also phosphorylate AMP-activated protein kinase (AMPK). This gene has its strongest expression in the brain and influences signalling cascades involved with learning and memory, neuronal differentiation and migration, neurite outgrowth, and synapse formation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. The identified isoforms differ in their ability to undergo autophosphorylation and to phosphorylate downstream kinases. [provided by RefSeq, Jul 2012],

Specifications

Size	50μL/100μL
Target	CaMKK2 Phospho Ser511
Reactivity	Human, Mouse, Rat
Applications	WB
MW(Calculated)
MW(Observed)	65kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	phosphate
Recommended Dilution Ratio	WB 1:1000-2000
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	Synthesized phosho peptide around human CaMKK2 (Ser511)
Specificity	This antibody detects endogenous levels of Human Mouse Rat CaMKK2 (phospho-Ser511)
Gene Name	CAMKK2 CAMKKB KIAA0787
Protein Name	Calcium/calmodulin-dependent protein kinase kinase 2
Other Name	CAMKK2; / CAMKKB; / KIAA0787; / Calcium/calmodulin-dependent protein kinase kinase 2; / CaM-KK 2; / CaM-kinase kinase 2; / CaMKK 2; / Calcium/calmodulin-dependent protein kinase kinase beta; / CaM-KK beta; / CaM-kinase kinase beta; / CaMKK beta
Species	Human
Gene ID	10645
Uniprot	Q96RR4,
Species.1	Mouse
Gene ID.1	207565
Uniprot.1	Q8C078,
Species.2	Rat
Gene ID.2	83506
Uniprot.2	O88831
Cellular Localization	Nucleus . Cytoplasm . Cell projection, neuron projection . Predominantly nuclear in unstimulated cells, relocalizes into cytoplasm and neurites after forskolin induction. .
Tissue Expression	Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Autophagy - animal;Organismal Systems >> Endocrine system >> Adipocytokine signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway
Research Areas	>>Autophagy - animal; / >>AMPK signaling pathway; / >>Longevity regulating pathway; / >>Adipocytokine signaling pathway; / >>Oxytocin signaling pathway; / >>Alcoholic liver disease; / >>Alcoholism
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Domain:The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.,Domain:The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.,enzyme regulation:Activated by Ca(2+)/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Autophosphorylation does not alter activity or regulation by Ca(2+)/calmodulin. In part, activity is independent on Ca(2+)/calmodulin.,Function:Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Seems to be involved in hippocampal activation of CREB1.,PTM:Autophosphorylated.,sequence Caution:Intron retention.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with calmodulin.,tissue specificity:Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.,