CaMKK2 Polyclonal Antibody
Ucallm_WP11395-50
Price range: $268.00 through $328.00
Product details
Background:
The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. The major isoform of this gene plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Protein products of this gene also phosphorylate AMP-activated protein kinase (AMPK). This gene has its strongest expression in the brain and influences signalling cascades involved with learning and memory, neuronal differentiation and migration, neurite outgrowth, and synapse formation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. The identified isoforms differ in their ability to undergo autophosphorylation and to phosphorylate downstream kinases. [provided by RefSeq, Jul 2012],
Specifications
| Size | 50μL/100μL |
| Target | CaMKK2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 65kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CAMKK2. AA range:381-430 |
| Specificity | CaMKIIβ Polyclonal Antibody detects endogenous levels of CaMKIIβ protein. |
| Gene Name | CAMKK2 CAMKKB KIAA0787 |
| Protein Name | Calcium/calmodulin-dependent protein kinase kinase 2 |
| Other Name | CAMKK2; / CAMKKB; / KIAA0787; / Calcium/calmodulin-dependent protein kinase kinase 2; / CaM-KK 2; / CaM-kinase kinase 2; / CaMKK 2; / Calcium/calmodulin-dependent protein kinase kinase beta; / CaM-KK beta; / CaM-kinase kinase beta; / CaMKK beta |
| Species | Human |
| Gene ID | 10645 |
| Uniprot | Q96RR4, |
| Species.1 | Mouse |
| Gene ID.1 | 207565 |
| Uniprot.1 | Q8C078, |
| Species.2 | Rat |
| Gene ID.2 | 83506 |
| Uniprot.2 | O88831 |
| Cellular Localization | Nucleus . Cytoplasm . Cell projection, neuron projection . Predominantly nuclear in unstimulated cells, relocalizes into cytoplasm and neurites after forskolin induction. . |
| Tissue Expression | Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Organismal Systems >> Endocrine system >> Adipocytokine signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway |
| Research Areas | >>Autophagy - animal; / >>AMPK signaling pathway; / >>Longevity regulating pathway; / >>Adipocytokine signaling pathway; / >>Oxytocin signaling pathway; / >>Alcoholic liver disease; / >>Alcoholism |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Domain:The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.,Domain:The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.,enzyme regulation:Activated by Ca(2+)/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Autophosphorylation does not alter activity or regulation by Ca(2+)/calmodulin. In part, activity is independent on Ca(2+)/calmodulin.,Function:Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Seems to be involved in hippocampal activation of CREB1.,PTM:Autophosphorylated.,sequence Caution:Intron retention.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with calmodulin.,tissue specificity:Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung., |
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