CAS Polyclonal Antibody
Ucallm_WP11412-50
Price range: $268.00 through $328.00
Product details
Background:
Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, the encoded protein may play a role both in apoptosis and in cell proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012],
Specifications
| Size | 50μL/100μL |
| Target | CAS |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IP, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 110kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IP 2-5 ug/mg lysate; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CSE1L. AA range:1-50 |
| Specificity | CAS Polyclonal Antibody detects endogenous levels of CAS protein. |
| Gene Name | CSE1L |
| Protein Name | Exportin-2 |
| Other Name | CSE1L; / CAS; / XPO2; / Exportin-2; / Exp2; / Cellular apoptosis susceptibility protein; / Chromosome segregation 1-like protein; / Importin-alpha re-exporter |
| Species | Human |
| Gene ID | 1434 |
| Uniprot | P55060, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q9ERK4 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Nucleus . Shuttles between the nucleus and the cytoplasm. . |
| Tissue Expression | Detected in brain, placenta, ovary, testis and trachea (at protein level) (PubMed:10331944). Widely expressed (PubMed:10331944). Highly expressed in testis and in proliferating cells (PubMed:7479798,PubMed:10331944). |
| Signaling_pathway | Genetic Information Processing >> Translation >> Nucleocytoplasmic transport |
| Research Areas | >>Nucleocytoplasmic transport; / >>Salmonella infection |
| Function | Function:Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.,similarity:Belongs to the XPO2/CSE1 family.,similarity:Contains 1 importin N-terminal domain.,subcellular location:Shuttles between the nucleus and the cytoplasm.,subunit:Found in a complex with CSE1L/XPO2, Ran and KPNA2. Binds with high affinity to importin-alpha only in the presence of RanGTP. The complex is dissociated by the combined action of RanBP1 and RanGAP1.,tissue specificity:Highly expressed in proliferating cells., |
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