SKU: WP11415 Categories: ,

Casein Kinase IIβ Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP11415-100
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Product details

Background:

This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013],

Specifications

Size50μL/100μL
TargetCasein Kinase IIβ
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)25kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human CKII-beta. AA range:166-215
SpecificityCasein Kinase IIβ Polyclonal Antibody detects endogenous levels of Casein Kinase IIβ protein.
Gene NameCSNK2B
Protein NameCasein kinase II subunit beta
Other NameCSNK2B; / CK2N; / G5A; / Casein kinase II subunit beta; / CK II beta; / Phosvitin; / Protein G5a
SpeciesHuman
Gene ID1460
UniprotP67870,
Species.1Mouse
Gene ID.113001
Uniprot.1P67871,
Species.2Rat
Gene ID.281650
Uniprot.2P67874
Cellular Localizationnucleus,nucleoplasm,cytoplasm,cytosol,plasma membrane,protein kinase CK2 complex,PcG protein complex,extracellular exosome,
Tissue ExpressionBrain,Epithelium,
Signaling_pathwayCellular Processes >> Transport and catabolism >> Mitophagy - animal;Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway
Research Areas>>Ribosome biogenesis in eukaryotes; / >>NF-kappa B signaling pathway; / >>Mitophagy - animal; / >>Wnt signaling pathway; / >>Adherens junction; / >>Alzheimer disease; / >>Prion disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Measles; / >>PD-L1 expression and PD-1 checkpoint pathway in cancer
FunctionFunction:Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit.,PTM:N-glycosylated.,PTM:Phosphorylated by alpha subunit.,similarity:Belongs to the casein kinase 2 subunit beta family.,similarity:Contains 1 UPAR/Ly6 domain.,subunit:Forms oligomer.,subunit:Tetramer composed of an alpha subunit, an alpha' subunit and two beta subunits. Interacts with TCTEX1D3 (By similarity). Interacts with CD163. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation.,
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