Name: Caspase-3 Polyclonal Antibody
SKU: WP11425
Price: 268 USD
Availability: InStock

Product details

Background:

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	Caspase-3
Reactivity	Human, Mouse, Rat, Fish
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	35kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; IF 1:100-300; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human Caspase 3. AA range:116-165
Specificity	Caspase-3 Polyclonal Antibody detects endogenous levels of Caspase-3 protein.
Gene Name	CASP3
Protein Name	Caspase3
Other Name	CASP3; / CPP32; / Caspase-3; / CASP-3; / Apopain; / Cysteine protease CPP32; / CPP-32; / Protein Yama; / SREBP cleavage activity 1; / SCA-1
Species	Human
Gene ID	836
Uniprot	P42574,
Species.1	Mouse
Gene ID.1	12367
Uniprot.1	P70677,
Species.2	Rat
Gene ID.2	12367
Uniprot.2	P55213
Cellular Localization	Cytoplasm.
Tissue Expression	Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Signaling_pathway	Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cell growth and death >> Apoptosis - multiple species;Cellular Processes >> Cell growth and death >> p53 signaling pathway;Organismal Systems >> Immune system >> Cytosolic DNA-sensing pathway;Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity;Organismal Systems >> Immune system >> IL-17 signaling pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Small cell lung cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Parkinson disease;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway
Research Areas	>>Platinum drug resistance; / >>MAPK signaling pathway; / >>p53 signaling pathway; / >>Apoptosis; / >>Apoptosis - multiple species; / >>Natural killer cell mediated cytotoxicity; / >>IL-17 signaling pathway; / >>TNF signaling pathway; / >>Serotonergic synapse; / >>Non-alcoholic fatty liver disease; / >>AGE-RAGE signaling pathway in diabetic complications; / >>Alcoholic liver disease; / >>Alzheimer disease; / >>Parkinson disease; / >>Amyotrophic lateral sclerosis; / >>Huntington disease; / >>Prion disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Epithelial cell signaling in Helicobacter pylori infection; / >>Pathogenic Escherichia coli infection; / >>Salmonella infection; / >>Pertussis; / >>Legionellosis; / >>Toxoplasmosis; / >>Amoebiasis; / >>Tuberculosis; / >>Hepatitis C; / >>Hepatitis B; / >>Measles; / >>Human cytomegalovirus infection; / >>Influenza A; / >>Human papillomavirus infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Herpes simplex virus 1 infection; / >>Epstein-Barr virus infection; / >>Human immunodeficiency virus 1 infection; / >>Pathways in cancer; / >>Viral carcinogenesis; / >>Proteoglycans in cancer; / >>MicroRNAs in cancer; / >>Colorectal cancer; / >>Small cell lung cancer; / >>Viral myocarditis; / >>Lipid and atherosclerosis
Function	Catalytic activity:Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-\|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.,enzyme regulation:Inhibited by isatin sulfonamides.,Function:Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-\|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.,PTM:Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.,PTM:S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.,similarity:Belongs to the peptidase C14A family.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.,tissue specificity:Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.,

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Caspase-3 Polyclonal Antibody

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Caspase-3 Polyclonal Antibody

Product details

Specifications

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