Name: Caspase-4 Polyclonal Antibody
SKU: WP11426
Price: 268 USD
Availability: InStock

Caspase-4 Polyclonal Antibody

Ucallm_WP11426-50

Price range: $268.00 through $328.00

Size

100μl

50μl

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SKU: WP11426-100

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Product details

Background:

This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	Caspase-4
Reactivity	Human
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	45kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human Caspase-4. AA range:75-124
Specificity	Caspase-4 Polyclonal Antibody detects endogenous levels of Caspase-4 protein.
Gene Name	CASP4 ICH2
Protein Name	Caspase4
Other Name	CASP4; / ICH2; / Caspase-4; / CASP-4; / ICE; / rel; / -II; / Protease ICH-2; / Protease TX
Species	Human
Gene ID	837
Uniprot	P49662
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasm, cytosol . Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side . Mitochondrion . Inflammasome . Secreted . Predominantly localizes to the endoplasmic reticulum (ER). Association with the ER membrane requires TMEM214 (PubMed:15123740). Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630). .
Tissue Expression	Widely expressed, including in keratinocytes and colonic and small intestinal epithelial cells (at protein level). Not detected in brain.
Signaling_pathway	Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway
Research Areas	>>Neutrophil extracellular trap formation; / >>NOD-like receptor signaling pathway; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Salmonella infection
Function	Catalytic activity:Strict requirement for Asp at the P1 position. It has a preferred cleavage sequence of Tyr-Val-Ala-Asp-\|- but also cleaves at Asp-Glu-Val-Asp-\|-.,Function:Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1.,PTM:The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8.,similarity:Belongs to the peptidase C14A family.,similarity:Contains 1 CARD domain.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit.,tissue specificity:Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain.,