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Home/Reagents/Antibody/Primary antibody/Polyclonal Antibody/Rabbit pAb/Caspase-7 Polyclonal Antibody
SKU: WP0025 Categories: ,

Caspase-7 Polyclonal Antibody

Ucallm_WP0025-50

Price range: $268.00 through $328.00

SKU: WP0025-100
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Product details

Background:

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],

Specifications

Size50μL/100μL
TargetCaspase-7
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)34kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-2000; IF 1:50-300; IHC 1:50-300; ELISA 1:5000-20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenSynthesized peptide derived from Caspase-7 . at AA range: 160-240
SpecificityCaspase-7 Polyclonal Antibody detects endogenous levels of Caspase-7 protein.
Gene NameCASP7
Protein NameCaspase7
Other NameCASP7; / MCH3; / Caspase-7; / CASP-7; / Apoptotic protease Mch-3; / CMH-1; / ICE-like apoptotic protease 3; / ICE-LAP3
SpeciesHuman
Gene ID840
UniprotP55210,
Species.1Mouse
Gene ID.1
Uniprot.1P97864
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationCytoplasm.
Tissue ExpressionHighly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
Signaling_pathwayCellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cell growth and death >> Apoptosis - multiple species;Organismal Systems >> Immune system >> Cytosolic DNA-sensing pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> TNF signaling pathway
Research Areas>>Apoptosis; / >>Apoptosis - multiple species; / >>TNF signaling pathway; / >>Non-alcoholic fatty liver disease; / >>Alzheimer disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Pathogenic Escherichia coli infection; / >>Salmonella infection; / >>Pertussis; / >>Legionellosis; / >>Pathways in cancer; / >>Lipid and atherosclerosis
FunctionCatalytic activity:Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Asp-Glu-Val-Asp-|-.,enzyme regulation:Inhibited by isatin sulfonamides.,Function:Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.,PTM:Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.,similarity:Belongs to the peptidase C14A family.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit.,tissue specificity:Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.,
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