Price range: $268.00 through $328.00
Background:
catalytic activity:Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity., function:Important for the overall degradation of proteins in lysosomes., similarity:Belongs to the peptidase C1 family., subunit:Dimer of a heavy and a light chain linked by disulfide bonds.,
| Size | 50μL/100μL |
| Target | Cathepsin L |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 25kD,37kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000-2000; IF 1:200-1:1000; ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human CATL1 (heavy chain, Cleaved-Ala114) |
| Specificity | This antibody detects endogenous levels of Human CATL1 (heavy chain, Cleaved-Ala114, protein was cleaved amino acid sequence between 113-114 ) |
| Gene Name | CTSL1 CTSL |
| Protein Name | CATL1 (heavy chain, Cleaved-Ala114) |
| Other Name | Cathepsin L1; / Major excreted protein; / MEP; / [Cleaved into: Cathepsin L1 heavy chain; / Cathepsin L1 light chain] |
| Species | Human |
| Gene ID | 1514 |
| Uniprot | P07711, |
| Species.1 | Mouse |
| Gene ID.1 | 13039 |
| Uniprot.1 | P06797, |
| Species.2 | Rat |
| Gene ID.2 | 25697 |
| Uniprot.2 | P07154 |
| Cellular Localization | Lysosome . Apical cell membrane; Peripheral membrane protein; Extracellular side . Cytoplasmic vesicle, secretory vesicle, chromaffin granule . Secreted, extracellular space . Secreted . Localizes to the apical membrane of thyroid epithelial cells. Released at extracellular space by activated dendritic cells and macrophages. .; [Isoform 2]: Nucleus . Translation initiation at downstream start sites allows the synthesis of isoforms that are devoid of a signal peptide and do not transit through the endoplasmic reticulum to localize to the nucleus (PubMed:15099520). Nuclear location varies during the cell cycle, with higher levels during S phase (PubMed:15099520). . |
| Tissue Expression | |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Phagosome;Cellular Processes >> Transport and catabolism >> Lysosome;Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cell growth and death >> Apoptosis;Organismal Systems >> Immune system >> Antigen processing and presentation;Human Diseases >> Immune disease >> Rheumatoid arthritis |
| Research Areas | >>Autophagy - animal; / >>Lysosome; / >>Phagosome; / >>Apoptosis; / >>Antigen processing and presentation; / >>Proteoglycans in cancer; / >>Rheumatoid arthritis; / >>Fluid shear stress and atherosclerosis |
| Function | proteolysis, |
