Cav2.2 Polyclonal Antibody
Ucallm_WP5894-50
Price range: $268.00 through $328.00
Product details
Background:
calcium voltage-gated channel subunit alpha1 B(CACNA1B) Homo sapiens The protein encoded by this gene is the pore-forming subunit of an N-type voltage-dependent calcium channel, which controls neurotransmitter release from neurons. The encoded protein forms a complex with alpha-2, beta, and delta subunits to form the high-voltage activated channel. This channel is sensitive to omega-conotoxin-GVIA and omega-agatoxin-IIIA but insensitive to dihydropyridines. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011],
Specifications
| Size | 50μL/100μL |
| Target | Cav2.2 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, IF |
| MW(Calculated) | |
| MW(Observed) | 263kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:50-100; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthetic Peptide of Cav2.2 AA range: 230-310 |
| Specificity | Cav2.2 protein(A205) detects endogenous levels of Cav2.2 |
| Gene Name | CACNA1B |
| Protein Name | Voltage-dependent N-type calcium channel subunit alpha-1B (Brain calcium channel III) (BIII) (Calcium channel, L type, alpha-1 polypeptide isoform 5) (Voltage-gated calcium channel subunit alpha Cav2.2) |
| Other Name | Voltage-dependent N-type calcium channel subunit alpha-1B; / Brain calcium channel III; / BIII; / Calcium channel, L type, alpha-1 polypeptide isoform 5; / Voltage-gated calcium channel subunit alpha Cav2.2 |
| Species | Human |
| Gene ID | 774 |
| Uniprot | Q00975, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | O55017, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q02294 |
| Cellular Localization | Membrane; Multi-pass membrane protein . |
| Tissue Expression | Isoform Alpha-1b-1 and isoform Alpha-1b-2 are expressed in the central nervous system, but not in skeletal muscle or aorta. Expressed in the cerebral white matter, cortex, hippocampus, basal ganglia, and cerebellum (PubMed:30982612). |
| Signaling_pathway | Organismal Systems >> Nervous system >> Cholinergic synapse;Organismal Systems >> Nervous system >> Dopaminergic synapse;Organismal Systems >> Nervous system >> Synaptic vesicle cycle;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway |
| Research Areas | >>MAPK signaling pathway; / >>Calcium signaling pathway; / >>Synaptic vesicle cycle; / >>Retrograde endocannabinoid signaling; / >>Cholinergic synapse; / >>Serotonergic synapse; / >>GABAergic synapse; / >>Dopaminergic synapse; / >>Type II diabetes mellitus; / >>Huntington disease; / >>Prion disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Morphine addiction; / >>Nicotine addiction; / >>Chemical carcinogenesis - receptor activation |
| Function | Domain:Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.,Function:Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1B gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by omega-conotoxin-GVIA (omega-CTx-GVIA) and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing alpha-1B subunit may play a role in directed migration of immature neurons.,PTM:Phosphorylated in vitro by CaM-kinase II, PKA, PKC and CGPK.,similarity:Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family.,similarity:Contains 1 EF-hand domain.,subunit:Multisubunit complex consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts with RIMS1 and RIMBP2.,tissue specificity:Isoform Alpha-1b-1 and isoform Alpha-1b-2 are expressed in the central nervous system, but not in skeletal muscle or aorta., |
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