Price range: $268.00 through $328.00
Background:
The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | CBP80 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 80kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NCBP1. AA range:1-50 |
| Specificity | CBP80 Polyclonal Antibody detects endogenous levels of CBP80 protein. |
| Gene Name | NCBP1 |
| Protein Name | Nuclear cap-binding protein subunit 1 |
| Other Name | NCBP1; / CBP80; / NCBP; / Nuclear cap-binding protein subunit 1; / 80 kDa nuclear cap-binding protein; / CBP80; / NCBP 80 kDa subunit |
| Species | Human |
| Gene ID | 4686 |
| Uniprot | Q09161, |
| Species.1 | Mouse |
| Gene ID.1 | 433702 |
| Uniprot.1 | Q3UYV9, |
| Species.2 | Rat |
| Gene ID.2 | 298075 |
| Uniprot.2 | Q56A27 |
| Cellular Localization | Nucleus . Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. . |
| Tissue Expression | Brain,Epithelium,Placenta, |
| Signaling_pathway | Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Genetic Information Processing >> Translation >> Nucleocytoplasmic transport |
| Research Areas | >>Nucleocytoplasmic transport; / >>mRNA surveillance pathway; / >>Spliceosome; / >>Amyotrophic lateral sclerosis |
| Function | Function:Involved in mediating U snRNA export from the nucleus. Binds to 5' capped mRNA.,similarity:Belongs to the NCBP1 family.,similarity:Contains 1 MIF4G domain.,subunit:Found in a U snRNA export complex with RNUXA/PHAX, CBP20, CBP80, RAN, XPO1 and m7G-capped RNA. Interaction with RNUXA/PHAX (By similarity). Heterodimer with NCBP2. Is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, UPF3B, UPF2, THOC4 and/or REFBP2., |
