Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010],
| Size | 50μL/100μL |
| Target | CD14 |
| Reactivity | Human |
| Applications | IHC, IF, FC, ELISA |
| MW(Calculated) | |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 17A2 |
| Immunogen | Purified recombinant fragment of human CD14 expressed in E. Coli. |
| Specificity | CD14 Monoclonal Antibody detects endogenous levels of CD14 protein. |
| Gene Name | CD14 |
| Protein Name | Monocyte differentiation antigen CD14 |
| Other Name | CD14; / Monocyte differentiation antigen CD14; / Myeloid cell-specific leucine-rich glycoprotein; / CD antigen CD14 |
| Species | Human |
| Gene ID | 929 |
| Uniprot | P08571, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P10810 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor . Secreted . Membrane raft . Golgi apparatus . Secreted forms may arise by cleavage of the GPI anchor. . |
| Tissue Expression | Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Phagosome;Organismal Systems >> Immune system >> Hematopoietic cell lineage;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway |
| Research Areas | >>MAPK signaling pathway; / >>NF-kappa B signaling pathway; / >>Phagosome; / >>Toll-like receptor signaling pathway; / >>Hematopoietic cell lineage; / >>Alcoholic liver disease; / >>Shigellosis; / >>Salmonella infection; / >>Pertussis; / >>Legionellosis; / >>Amoebiasis; / >>Tuberculosis; / >>Transcriptional misregulation in cancer; / >>Acute myeloid leukemia; / >>Lipid and atherosclerosis |
| Function | Function:Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.,online information:CD14 entry,similarity:Contains 11 LRR (leucine-rich) repeats.,subunit:Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.,tissue specificity:Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages., |
