Price range: $268.00 through $328.00
Background:
CD20 is a B cell specific membrane protein, which is mainly distributed in B cells, pre-B cells, post-B cells and B cells before plasma cells. CD20 antibody generally does not cross react with T cells, and has good cell specificity for B-cell lymphoma and acute and chronic lymphocytic leukemia. Therefore, CD20 is the most commonly used B-cell marker in the classification of malignant lymphoma, especially T or B-cell lymphoma.
| Target | CD20 |
| Reactivity | Human |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 33kD |
| MW(Observed) | 35kD |
| Host Species | Mouse |
| Isotype | IgG1,Kappa |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Source | |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | ABT065 |
| Immunogen | Synthesized peptide derived from human CD20 AA range: 200-297 |
| Sequence | |
| Specificity | The antibody can specifically recognize human CD20 protein. |
| Gene Name | MS4A1 CD20 |
| Protein Name | CD20 |
| Other Name | APY; / ATOPY; / B lymphocyte antigen CD20; / B Lymphocyte Cell Surface Antigen B1; / B-lymphocyte antigen CD20; / B-lymphocyte cell-surface antigen B1; / B-lymphocyte surface antigen B1; / B1; / Bp 35; / Bp35; / CD 20; / CD20; / CD20 antigen; / CD20 receptor; / CD20_HUMAN; / CVID 5; / CVID5; / Fc epsilon receptor I beta chain; / Fc Fragment of IgE high affinity I receptor for beta polypeptide; / FCER1B; / High affinity immunoglobulin epsilon receptor subunit beta; / IgE Fc receptor subunit beta; / IGEL; / IGER; / IGHER; / LEU 16; / Leu-16; / LEU16; / leukocyte surface antigen Leu 16; / Leukocyte surface antigen Leu-16; / Ly44; / Membrane spanning 4 domains A1; / Membrane spanning 4 domains subfamily A member 2; / membrane-spanning 4-domains A1; / Membrane-spanning 4-domains subfamily A member 1; / MGC3969; / MS4A1; / MS4A2; / S7 |
| Species | Human |
| Gene ID-1 | 931 |
| Uniprot | P11836 |
| Species.1 | |
| Gene ID-2 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID-3 | |
| Uniprot.2 | |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | CD20 is a B cell specific membrane protein, which is mainly distributed in B cells, pre-B cells, post-B cells and B cells before plasma cells. CD20 antibody generally does not cross react with T cells, and has good cell specificity for B-cell lymphoma and acute and chronic lymphocytic leukemia. Therefore, CD20 is the most commonly used B-cell marker in the classification of malignant lymphoma, especially T or B-cell lymphoma. |
| Cellular Localization | Membranous |
| Tissue Expression | Expressed on B-cells. |
| Signaling_pathway | Organismal Systems >> Immune system >> Hematopoietic cell lineage |
| Research Areas | >>Hematopoietic cell lineage |
| Function | Caution:Epitope 1, mapped in PubMed:16785532, is predicted to be buried in the membrane. Its accessibility to the extracellular space, and thus to antibody recognition, is not explained.,Function:This protein may be involved in the regulation of B-cell activation and proliferation.,online information:CD20 entry,pharmaceutical:Monoclonal antibodies(mAb) against CD20 are used to treat B-cell non-Hodgkin's lymphoma (NHL). These antibodies include Rituximab (Mabthera), Britumomab (Zevalin) and Tositumomab (Bexxar). CD20 engaged by mAb can generate transmembrane signals capable of directly controlling cell growth and triggering cell death in certain tumors. Alternatively, mAb can mediate complement-dependent cytotoxicity.,PTM:Phosphorylated. Might be functionally regulated by protein kinase(s).,similarity:Belongs to the MS4A family.,tissue specificity:Expressed on B-cells., |
| RRID |
