CD247 Monoclonal Antibody [4B10]
Ucallm_WM0091-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | CD3 ζ |
| Reactivity | Human |
| Applications | WB, IHC, IF, FC, ELISA |
| MW(Calculated) | 19kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 4B10 |
| Immunogen | Purified recombinant fragment of human CD247 expressed in E. Coli. |
| Specificity | CD247 Monoclonal Antibody detects endogenous levels of CD247 protein. |
| Gene Name | CD247 |
| Protein Name | T-cell surface glycoprotein CD3 zeta chain |
| Other Name | CD247; / CD3Z; / T3Z; / TCRZ; / T-cell surface glycoprotein CD3 zeta chain; / T-cell receptor T3 zeta chain; / CD antigen CD247 |
| Species | Human |
| Gene ID | 919 |
| Uniprot | P20963 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. |
| Tissue Expression | CD3Z is expressed in normal lymphoid tissue and in peripheral blood mononuclear cells (PBMCs) (PubMed:11722641). |
| Signaling_pathway | Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Th1 and Th2 cell differentiation;Organismal Systems >> Immune system >> Th17 cell differentiation;Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer |
| Research Areas | >>Natural killer cell mediated cytotoxicity; / >>Th1 and Th2 cell differentiation; / >>Th17 cell differentiation; / >>T cell receptor signaling pathway; / >>Chagas disease; / >>Epstein-Barr virus infection; / >>Human immunodeficiency virus 1 infection; / >>PD-L1 expression and PD-1 checkpoint pathway in cancer |
| Function | Disease:Defects in CD247 are a cause of primary T-cell immunodeficiency [MIM:610163]. Affected individuals suffer of recurrent infections. Patients T-cell counts are very low and B-cell counts are normal.,Domain:The ITAM domains mediate interaction with SHB.,Function:Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.,online information:CD247 mutation db,PTM:Phosphorylated on Tyr residues after T-cell receptor triggering.,similarity:Belongs to the CD3Z/FCER1G family.,similarity:Contains 3 ITAM domains.,subunit:The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3-zeta forms either homodimers or heterodimers with CD3-eta. Interacts with SLA and SLA2. Interacts with DOCK2 and TRAT1. Interacts with HIV-1 Nef protein. Interacts with SHB., |
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