SKU: WP15633 Categories: ,

CD249 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP15633-100
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Product details

Background:

catalytic activity:Release of N-terminal glutamate (and to a lesser extent aspartate) from a peptide., cofactor:Binds 1 zinc ion per subunit., function:Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells., similarity:Belongs to the peptidase M1 family., subunit:Homodimer; disulfide-linked., tissue specificity:Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.,

Specifications

Size50μL/100μL
TargetCD249
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)110kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC: 1:100-1:300; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human ENPEP. AA range:671-720
SpecificityCD249 Polyclonal Antibody detects endogenous levels of CD249 protein.
Gene NameENPEP
Protein NameGlutamyl aminopeptidase
Other NameENPEP; / Glutamyl aminopeptidase; / EAP; / Aminopeptidase A; / AP-A; / Differentiation antigen gp160; / CD249
SpeciesHuman
Gene ID2028
UniprotQ07075,
Species.1Mouse
Gene ID.113809
Uniprot.1P16406,
Species.2Rat
Gene ID.264017
Uniprot.2P50123
Cellular LocalizationCell membrane; Single-pass type II membrane protein.
Tissue ExpressionExpressed in choriocarcinoma cancer cell lines (at protein level) (PubMed:10692253). Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.
Signaling_pathwayOrganismal Systems >> Endocrine system >> Renin-angiotensin system
Research Areas>>Renin-angiotensin system
FunctionCatalytic activity:Release of N-terminal glutamate (and to a lesser extent aspartate) from a peptide.,cofactor:Binds 1 zinc ion per subunit.,Function:Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.,similarity:Belongs to the peptidase M1 family.,subunit:Homodimer; disulfide-linked.,tissue specificity:Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.,
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