Price range: $268.00 through $328.00
Background:
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011],
| Size | 50μL/100μL |
| Target | CD34 |
| Reactivity | Human |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 41kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 16A9 |
| Immunogen | Purified recombinant fragment of CD34 expressed in E. Coli. |
| Specificity | CD34 Monoclonal Antibody detects endogenous levels of CD34 protein. |
| Gene Name | CD34 |
| Protein Name | Hematopoietic progenitor cell antigen CD34 |
| Other Name | CD34; / Hematopoietic progenitor cell antigen CD34; / CD antigen CD34 |
| Species | Human |
| Gene ID | 947 |
| Uniprot | P28906, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q64314 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Membrane; Single-pass type I membrane protein. |
| Tissue Expression | Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. |
| Signaling_pathway | Organismal Systems >> Immune system >> Hematopoietic cell lineage;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules |
| Research Areas | >>Cell adhesion molecules; / >>Hematopoietic cell lineage |
| Function | Alternative products:Both isoforms are expressed on the cell surface. CD34-T/CD34-F ratio increases with cell differentiation,developmental stage:On early hematopoietic progenitor cells.,Disease:Abnormal CD34 expression in leukemogenesis.,Function:Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.,online information:CD34 entry,PTM:Highly glycosylated.,PTM:Phosphorylated on serine residues by PKC.,similarity:Belongs to the CD34 family.,tissue specificity:Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues., |
