Name: CD44 [ABT476] mouse mAb
SKU: WM1501
Price: 268 USD
Availability: InStock

Product details

Background:
CD44 is a cell surface receptor, which belongs to the family of cell adhesion molecules that mediate cell-to-cell and cell-to-matrix adhesion, and interacts with cytoskeletal proteins. CD44 is widely expressed in hematopoietic cells and mesodermal cells and is a marker of these cells. Studies have shown that the loss of CD44 expression is related to invasion, metastasis and progression of lung cancer, prostate cancer, breast cancer, ovarian cancer and malignant melanoma.

Specifications

Target	CD44
Reactivity	Human, Mouse, Rat
Applications	IHC, WB, IF, ELISA
MW(Calculated)	81kD
MW(Observed)	81kD
Host Species	Mouse
Isotype	IgG1,Kappa
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	ABT476
Immunogen	Synthesized peptide derived from human CD44 AA range: 100-200
Sequence
Specificity	The antibody can specifically recognize human CD44 protein. In western blotting of Hela cell lysate, the antibody can label a 81 kDa band corresponding to CD44.
Gene Name	CD44 LHR MDU2 MDU3 MIC4
Protein Name	CD44
Other Name	CD44 antigen; / CDw44; / Epican; / Extracellular matrix receptor III; / ECMR-III; / GP90 lymphocyte homing/adhesion receptor; / HUTCH-I; / Heparan sulfate proteoglycan; / Hermes antigen; / Hyaluronate receptor; / Phagocytic glycoprotein 1; / PGP-1; / Phagocytic glycoprotein I; / PGP-I; / CD antigen CD44
Species	Human
Gene ID-1	960
Uniprot	P16070
Species.1
Gene ID-2
Uniprot.1
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	CD44 is a cell surface receptor, which belongs to the family of cell adhesion molecules that mediate cell-to-cell and cell-to-matrix adhesion, and interacts with cytoskeletal proteins. CD44 is widely expressed in hematopoietic cells and mesodermal cells and is a marker of these cells. Studies have shown that the loss of CD44 expression is related to invasion, metastasis and progression of lung cancer, prostate cancer, breast cancer, ovarian cancer and malignant melanoma.
Cellular Localization	Membranous
Tissue Expression	Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Signaling_pathway	Organismal Systems >> Immune system >> Hematopoietic cell lineage;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Environmental Information Processing >> Signaling molecules and interaction >> ECM-receptor interaction
Research Areas	>>ECM-receptor interaction; / >>Hematopoietic cell lineage; / >>Shigellosis; / >>Epstein-Barr virus infection; / >>Proteoglycans in cancer; / >>MicroRNAs in cancer
Function	Alternative products:Additional isoforms seem to exist. Additional isoforms are produced by alternative splicing of 10 out of 19 exons within the extracellular domain. Additional diversity is generated through the utilization of internal splice donor and acceptor sites within 2 of the exons. A variation in the cytoplasmic domain was shown to result from the alternative splicing of 2 exons. Isoform CD44 is expected to be expressed in normal cells. Splice variants have been found in many tumor cell lines. Exons 5, 6, 7, 8, 9, 10, 11, 13, 14 and 19 are alternatively spliced. Experimental confirmation may be lacking for some isoforms,Function:Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.,online information:Blood group antigen gene mutation database,online information:CD44 entry,polymorphism:CD44 is responsible for the Indian blood group system. The molecular basis of the In(A)=In1/In(B)=In2 blood group antigens is a single variation in position 46; In(B), the most frequent allele, has Arg-46.,PTM:N-glycosylated.,PTM:O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).,PTM:Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.,PTM:Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.,similarity:Contains 1 Link domain.,subunit:Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment.,tissue specificity:An epithelial isoform (CD44E) is expressed by cells of epithelium and highly expressed by carcinomas. An hematopoietic isoform (CD44H) is expressed by cells of mesodermal origin. Expression is repressed in neuroblastoma cells.,
RRID

Please login to post questions

CD44 [ABT476] mouse mAb

Product details

Specifications

About Us

Support

Order

Menu

CD44 [ABT476] mouse mAb

Product details

Specifications

Related products

U-Blot® NLRP3 Rabbit mAb

U-Blot® HDAC2 Rabbit mAb

U-Blot™ Phosphate Buffered Saline with Tween 20 (PBS-T), pH 7.4

U-Blot® Smad2 (Phospho Ser250) Rabbit mAb

U-Blot® GAPDH Rabbit mAb

U-Blot® α smooth muscle actin Rabbit mAb

Customer Reviews

Menu