Price range: $268.00 through $328.00
Background:
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015],
| Size | 50μL/100μL |
| Target | CD71/TfR |
| Reactivity | Human |
| Applications | WB, IHC, IF, FC, ELISA |
| MW(Calculated) | 85kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 17F6 |
| Immunogen | Purified recombinant fragment of human CD71 expressed in E. Coli. |
| Specificity | CD71 Monoclonal Antibody detects endogenous levels of CD71 protein. |
| Gene Name | TFR1 |
| Protein Name | Transferrin receptor protein 1 |
| Other Name | TFRC; / Transferrin receptor protein 1; / TR; / TfR; / TfR1; / Trfr; / T9; / p90; / CD antigen CD71 |
| Species | Human |
| Gene ID | 4155 |
| Uniprot | P02786, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q62351 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .; [Transferrin receptor protein 1, serum form]: Secreted . |
| Tissue Expression | Brain,Epithelium,Erythroleukemia,Eye,Human endometrium carcinoma cell line,Liver,Pl |
| Signaling_pathway | |
| Research Areas | >>HIF-1 signaling pathway; / >>Endocytosis; / >>Phagosome; / >>Ferroptosis; / >>Hematopoietic cell lineage |
| Function | Function:Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.,induction:Regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation.,miscellaneous:Canine and feline parvoviruses bind human and feline transferrin receptors and use these receptors to enter and infect cells.,miscellaneous:Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemia resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.,PTM:N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.,PTM:Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.,PTM:Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).,similarity:Belongs to the peptidase M28 family. M28B subfamily.,similarity:Contains 1 PA (protease associated) domain.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with Machupo arenavirus GPC., |
