Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011],
| Size | 50μL/100μL |
| Target | CD73 |
| Reactivity | Human |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 2B9 |
| Immunogen | Purified recombinant fragment of CD73 expressed in E. Coli. |
| Specificity | CD73 Monoclonal Antibody detects endogenous levels of CD73 protein. |
| Gene Name | NT5E |
| Protein Name | 5'-nucleotidase |
| Other Name | NT5E; / NT5; / NTE; / 5'-nucleotidase; / 5'-NT; / Ecto-5'-nucleotidase; / CD antigen CD73 |
| Species | Human |
| Gene ID | 4907 |
| Uniprot | P21589, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q61503 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor . |
| Tissue Expression | Leukocyte,Liver,Placenta,Skin, |
| Signaling_pathway | |
| Research Areas | >>Purine metabolism; / >>Pyrimidine metabolism; / >>Nicotinate and nicotinamide metabolism; / >>Metabolic pathways; / >>Nucleotide metabolism |
| Function | Catalytic activity:A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate.,cofactor:Zinc.,Disease:There is a decrease in the activity of NT5 in B-cell chronic lymphocytic leukemia.,Function:Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.,similarity:Belongs to the 5'-nucleotidase family.,subunit:Homodimer; disulfide-linked., |
