U-Blot® CD9 Rabbit mAb
WR4307 Western blot analysis of lysates from Rat womb cells, using CD9 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011],
Specifications
| Size | 50μL/100μL |
| Target | CD9 |
| Reactivity | Human, Mouse, Rat, Bovine |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 25kD |
| MW(Observed) | 20kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0507R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | CD9 |
| Protein Name | CD9 antigen |
| Other Name | CD9; / MIC3; / TSPAN29; / GIG2; / CD9 antigen; / 5H9 antigen; / Cell growth-inhibiting gene 2 protein; / Leukocyte antigen MIC3; / Motility-related protein; / MRP-1; / Tetraspanin-29; / Tspan-29; / p24; / CD antigen CD9 |
| Species | Human |
| Gene ID | 928 |
| Uniprot | P21926, |
| Species.1 | Mouse |
| Gene ID.1 | 12527 |
| Uniprot.1 | P40240, |
| Species.2 | Rat |
| Gene ID.2 | 24936 |
| Uniprot.2 | P40241 |
| Cellular Localization | Membrane |
| Tissue Expression | Detected in platelets (at protein level) (PubMed:19640571). Expressed by a variety of hematopoietic and epithelial cells (PubMed:19640571). |
| Signaling_pathway | Organismal Systems >> Immune system >> Hematopoietic cell lineage |
| Research Areas | >>Hematopoietic cell lineage |
| Function | Function:Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.,PTM:Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.,similarity:Belongs to the tetraspanin (TM4SF) family.,subunit:Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8.,tissue specificity:Expressed by a variety of hematopoietic and epithelial cells., |
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