Cdc6 Polyclonal Antibody
Ucallm_WP11547-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | Cdc6 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 60kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CDC6. AA range:491-540 |
| Specificity | Cdc6 Polyclonal Antibody detects endogenous levels of Cdc6 protein. |
| Gene Name | CDC6 |
| Protein Name | Cell division control protein 6 homolog |
| Other Name | CDC6; / CDC18L; / Cell division control protein 6 homolog; / CDC6-related protein; / Cdc18-related protein; / HsCdc18; / p62; / cdc6; / HsCDC6 |
| Species | Human |
| Gene ID | 990 |
| Uniprot | Q99741, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | O89033 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Cytoplasm . The protein is nuclear in G1 and cytoplasmic in S-phase cells (PubMed:9566895). . |
| Tissue Expression | Brain,Epithelium, |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Cell cycle |
| Research Areas | >>Cell cycle; / >>Chemical carcinogenesis - receptor activation |
| Function | Function:Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.,similarity:Belongs to the CDC6/cdc18 family.,subcellular location:The protein is nuclear in G1 and cytoplasmic in S-phase cells.,subunit:Interacts with PCNA, ORC1L, cyclin-CDK and HUWE1., |
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