Cofilin Polyclonal Antibody
Ucallm_WP11706-50
Price range: $268.00 through $328.00
Product details
Background:
cofilin 1(CFL1) Homo sapiens The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004],
Specifications
| Size | 50μL/100μL |
| Target | Cofilin |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 19kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Cofilin. AA range:61-110 |
| Specificity | Cofilin Polyclonal Antibody detects endogenous levels of Cofilin protein. |
| Gene Name | CFL1/CFL2 |
| Protein Name | Cofilin-1/2 |
| Other Name | CFL1; / CFL; / Cofilin-1; / 18 kDa phosphoprotein; / p18; / Cofilin; / non-muscle isoform; / CFL2; / Cofilin-2; / Cofilin, muscle isoform |
| Species | Human |
| Gene ID | 1073;1072 |
| Uniprot | P23528;Q9Y281, |
| Species.1 | Mouse |
| Gene ID.1 | 12631;12632, |
| Uniprot.1 | |
| Species.2 | Rat |
| Gene ID.2 | 29271 |
| Uniprot.2 | P45592 |
| Cellular Localization | Nucleus matrix . Cytoplasm, cytoskeleton . Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side . Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side . Cell projection, lamellipodium . Cell projection, growth cone . Cell projection, axon . Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. |
| Tissue Expression | Widely distributed in various tissues. |
| Signaling_pathway | Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis |
| Research Areas | >>Axon guidance; / >>Fc gamma R-mediated phagocytosis; / >>Regulation of actin cytoskeleton; / >>Pertussis; / >>Human immunodeficiency virus 1 infection |
| Function | Function:Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.,online information:Cofilin entry,PTM:Phosphorylated on Ser-3 in resting cells.,similarity:Belongs to the actin-binding proteins ADF family.,similarity:Contains 1 ADF-H domain.,subcellular location:Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.,tissue specificity:Widely distributed in various tissues., |
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