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Home/Reagents/Antibody/Primary antibody/Monoclonal Antibody/Rabbit mAb/U-Blot® Cofilin Rabbit mAb
SKU: WR4465 Categories: ,

U-Blot® Cofilin Rabbit mAb

Western Blot analysis using C6 lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(WS0446)was diluted at 1:10000, 37° 1hour.
WR4465 Western Blot analysis using C6 lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(WS0446)was diluted at 1:10000, 37° 1hour.

Price range: $268.00 through $328.00

SKU: WR4465-50
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Product details

Background:cofilin 1(CFL1) Homo sapiens The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004],

Specifications

Size50μL/100μL
TargetCofilin
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)19kD
MW(Observed)19kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:20000-1:50000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM0685R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NameCFL1 CFL
Protein NameCofilin
Other NameCofilin-1; / 18 kDa phosphoprotein; / p18; / Cofilin, non-muscle isoform
SpeciesHuman
Gene ID1072
UniprotP23528,
Species.1Mouse
Gene ID.112631
Uniprot.1P18760,
Species.2Rat
Gene ID.229271
Uniprot.2P45592
Cellular LocalizationNucleus matrix . Cytoplasm, cytoskeleton . Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side . Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side . Cell projection, lamellipodium . Cell projection, growth cone . Cell projection, axon . Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
Tissue ExpressionWidely distributed in various tissues.
Signaling_pathwayCellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis
Research Areas>>Axon guidance; / >>Fc gamma R-mediated phagocytosis; / >>Regulation of actin cytoskeleton; / >>Pertussis; / >>Human immunodeficiency virus 1 infection
FunctionFunction:Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.,online information:Cofilin entry,PTM:Phosphorylated on Ser-3 in resting cells.,similarity:Belongs to the actin-binding proteins ADF family.,similarity:Contains 1 ADF-H domain.,subcellular location:Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.,tissue specificity:Widely distributed in various tissues.,
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