COL12A1 Polyclonal Antibody
Ucallm_WP11709-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes the alpha chain of type XII collagen, a member of the FACIT (fibril-associated collagens with interrupted triple helices) collagen family. Type XII collagen is a homotrimer found in association with type I collagen, an association that is thought to modify the interactions between collagen I fibrils and the surrounding matrix. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | COL12A1 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | 333kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Collagen XII alpha1. AA range:1481-1530 |
| Specificity | COL12A1 Polyclonal Antibody detects endogenous levels of COL12A1 protein. |
| Gene Name | COL12A1 |
| Protein Name | Collagen alpha-1(XII) chain |
| Other Name | COL12A1; / COL12A1L; / Collagen alpha-1; / XII; / chain |
| Species | Human |
| Gene ID | 1303 |
| Uniprot | Q99715, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q60847 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Secreted, extracellular space, extracellular matrix . |
| Tissue Expression | Found in collagen I-containing tissues: both isoform 1 and isoform 2 appear in amnion, chorion, skeletal muscle, small intestine, and in cell culture of dermal fibroblasts, keratinocytes and endothelial cells. Only isoform 2 is found in lung, placenta, kidney and a squamous cell carcinoma cell line. Isoform 1 is also present in the corneal epithelial Bowman's membrane (BM) and the interfibrillar matrix of the corneal stroma, but it is not detected in the limbal BM. |
| Signaling_pathway | |
| Research Areas | >>Protein digestion and absorption |
| Function | Alternative products:The final tissue form of collagen XII may contain homotrimers of either isoform 1 or isoform 2 or any combination of isoform 1 and isoform 2,Function:Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.,PTM:Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.,PTM:O-glycosylation of isoform 1; glycosaminoglycan of chondroitin-sulfate type.,PTM:The triple-helical tail is stabilized by disulfide bonds at each end.,similarity:Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.,similarity:Contains 1 TSP N-terminal (TSPN) domain.,similarity:Contains 18 fibronectin type-III domains.,similarity:Contains 4 VWFA domains.,subunit:Trimer of identical chains each containing 190 kDa of non-triple-helical sequences.,tissue specificity:Found in collagen I-containing tissues: both isoform 1 and isoform 2 appear in amnion, chorion, skeletal muscle, small intestine, and in cell culture of dermal fibroblasts, keratinocytes and endothelial cells. Only isoform 2 is found in lung, placenta, kidney and a squamous cell carcinoma cell line. Isoform 1 is also present in the corneal epithelial Bowman's membrane (BM) and the interfibrillar matrix of the corneal stroma, but it is not detected in the limbal BM., |
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