Name: U-Blot® Cortactin Rabbit mAb
SKU: WR4839
Price: 268 USD
Availability: InStock

U-Blot® Cortactin Rabbit mAb

Ucallm_WR4839-50

Price range: $268.00 through $328.00

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100μl

50μl

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SKU: WR4839-50

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Product details

Background:cortactin(CTTN) Homo sapiens This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010],

Specifications

Size	50μL/100μL
Target	Cortactin
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	62kD
MW(Observed)	80kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0960R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	CTTN
Protein Name	Src substrate cortactin
Other Name	CTTN; / EMS1; / Src substrate cortactin; / Amplaxin; / Oncogene EMS1
Species	Human
Gene ID	13043
Uniprot	Q14247,
Species.1	Mouse
Gene ID.1	13043
Uniprot.1	Q60598
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasm, cytoskeleton . Cell projection, lamellipodium . Cell projection, ruffle. Cell projection, dendrite . Cell projection . Cell membrane; Peripheral membrane protein; Cytoplasmic side . Cell projection, podosome . Cell junction . Cell junction, focal adhesion . Membrane, clathrin-coated pit . Cell projection, dendritic spine . Cytoplasm, cell cortex . Colocalizes transiently with PTK2/FAK1 at focal adhesions (By similarity). Associated with membrane ruffles and lamellipodia. In the presence of CTTNBP2NL, colocalizes with stress fibers (By similarity). In the presence of CTTNBP2, localizes at the cell cortex (By similarity). In response to neuronal activation by glutamate, redistributes from dendritic spines to the dendritic shaft (By similarity). Colocalizes with DNM2 at the basis of filopodia in hippocampus neuron growth zones (By similarity). .
Tissue Expression	Coronary artery,Epithelium,Mammary gland,Placenta,Platelet,Testis,
Signaling_pathway
Research Areas	>>Tight junction; / >>Bacterial invasion of epithelial cells; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Proteoglycans in cancer
Function	Function:May contribute to the organization of cell structure. The SH3 motif may function as a binding region to cytoskeleton. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.,online information:Cortactin entry,similarity:Contains 1 SH3 domain.,similarity:Contains 7 cortactin repeats.,subcellular location:Associated with membrane ruffles and lamellipodia.,subunit:Interacts with SHANK2 and SHANK3 via its SH2 domain. Also interacts with FGD1 (By similarity). Interacts with PLXDC2.,