Price range: $268.00 through $328.00
Background:
COX has two isomeric forms of COX-1 and COX-2. It is a membrane-bound protease involved in the conversion of arachidonic acid to prostaglandins. COX-2 is an inducible enzyme that participates in the expression of cell growth factors, tumor promoting factors and cytokines, and is highly expressed in breast, lung, and colon cancers. It is mainly used in tumor research and is one of the targets of tumor therapy.
| Size | 50μL/100μL |
| Target | COX2 |
| Reactivity | Human |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 69kD |
| MW(Observed) | 70kD |
| Host Species | Mouse |
| Isotype | IgG2b,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM-COX2 |
| Immunogen | Synthesized peptide derived from human COX-2 (cyclooxygenase-2) AA range: 500-604 |
| Specificity | The antibody can specifically recognize human COX-2 protein. In western blotting of Hela and A549 cell lysates, the antibody can label a 70 kDa band corresponding to COX-2. |
| Gene Name | PTGS2 COX2 |
| Protein Name | Prostaglandin G/H synthase 2 (Cyclooxygenase-2) (COX-2) (PHS II) (Prostaglandin H2 synthase 2) (PGH synthase 2) (PGHS-2) (Prostaglandin-endoperoxide synthase 2) |
| Other Name | COX 2; / COX-2; / COX2; / Cyclooxygenase 2; / Cyclooxygenase 2b; / Cyclooxygenase; / Cyclooxygenase-2; / Cyclooxygenase2; / fj02a10; / Glucocorticoid-regulated inflammatory cyclooxygenase; / Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase; / GRIPGHS; / hCox 2; / Macrophage activation-associated marker protein P71/73; / OTTHUMP00000033524; / PES-2; / PGG/HS; / PGH synthase 2; / PGH2_HUMAN; / PGHS 2; / PGHS-2; / PGHS2; / PHS 2; / PHS II; / PHS2; / Prostaglandin endoperoxide synthase 2; / prostaglandin G/H synthase and cyclooxygenase; / Prostaglandin endoperoxide synthase 2; / Prostaglandin G/H synthase 2; / Prostaglandin G/H synthase 2 precursor; / Prostaglandin G/H synthase and cyclooxygenase; / Prostaglandin G/H synthase; / Prostaglandin H2 synthase 2; / prostaglandin-endoperoxide synthase 2; / prostaglandin G/H synthase and cyclooxygenase; / Prostaglandin-endoperoxide synthase 2; / PTGS2; / ptgs2a; / TIS10; / TIS10 protein; / unp1239; / wu:fj02a10 |
| Species | Human |
| Gene ID | 5743 |
| Uniprot | P35354 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasmic |
| Tissue Expression | Endothelial cell,Epidermal keratinocytes in primary culture,Lung,Pe |
| Signaling_pathway | Organismal Systems >> Immune system >> IL-17 signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: specific types >> Small cell lung cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway |
| Research Areas | >>Arachidonic acid metabolism; / >>Metabolic pathways; / >>NF-kappa B signaling pathway; / >>VEGF signaling pathway; / >>C-type lectin receptor signaling pathway; / >>IL-17 signaling pathway; / >>TNF signaling pathway; / >>Retrograde endocannabinoid signaling; / >>Serotonergic synapse; / >>Ovarian steroidogenesis; / >>Oxytocin signaling pathway; / >>Regulation of lipolysis in adipocytes; / >>Alzheimer disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Leishmaniasis; / >>Human cytomegalovirus infection; / >>Human papillomavirus infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Pathways in cancer; / >>Chemical carcinogenesis - DNA adducts; / >>MicroRNAs in cancer; / >>Small cell lung cancer |
| Function | Catalytic activity:Arachidonate + AH(2) + 2 O(2) = prostaglandin H(2) + A + H(2)O.,cofactor:Binds 1 heme B (iron-protoporphyrin IX) group per subunit.,Disease:Likely to play a role in inflammatory diseases such as rheumatoid arthritis.,Function:May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.,induction:By cytokines and mitogens.,miscellaneous:This enzyme acts both as a dioxygenase and as a peroxidase.,miscellaneous:This enzyme is the target of nonsteroidal anti-inflammatory drugs such as aspirin.,pathway:Lipid metabolism; prostaglandin biosynthesis.,similarity:Belongs to the prostaglandin G/H synthase family.,similarity:Contains 1 EGF-like domain.,subunit:Homodimer., |
