SKU: WP11772 Categories: ,

COX5b Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP11772-100
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Product details

Background:

Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetCOX5b
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)14kD
MW(Observed)
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:100-1:300; WB 1:1000-1:5000; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human COX5B. AA range:11-60
SpecificityCOX5b Polyclonal Antibody detects endogenous levels of COX5b protein.
Gene NameCOX5B
Protein NameCytochrome c oxidase subunit 5B mitochondrial
Other NameCOX5B; / Cytochrome c oxidase subunit 5B; / mitochondrial; / Cytochrome c oxidase polypeptide Vb
SpeciesHuman
Gene ID1329
UniprotP10606,
Species.1Mouse
Gene ID.1
Uniprot.1P19536,
Species.2Rat
Gene ID.294194
Uniprot.2P12075
Cellular LocalizationMitochondrion inner membrane; Peripheral membrane protein; Matrix side .
Tissue ExpressionLiver,Skin,
Signaling_pathwayOrganismal Systems >> Circulatory system >> Cardiac muscle contraction;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Parkinson disease;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases
Research Areas>>Oxidative phosphorylation; / >>Metabolic pathways; / >>Cardiac muscle contraction; / >>Thermogenesis; / >>Non-alcoholic fatty liver disease; / >>Alzheimer disease; / >>Parkinson disease; / >>Amyotrophic lateral sclerosis; / >>Huntington disease; / >>Prion disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Chemical carcinogenesis - reactive oxygen species; / >>Diabetic cardiomyopathy
FunctionFunction:This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.,similarity:Belongs to the cytochrome c oxidase subunit 5B family.,
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