cPLA2-ε Polyclonal Antibody
Ucallm_WP11782-50
Price range: $268.00 through $328.00
Product details
Background:
catalytic activity:Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate., domain:The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+)., enzyme regulation:Stimulated by cytosolic Ca(2+)., function:Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position., similarity:Contains 1 C2 domain., similarity:Contains 1 PLA2c domain., subcellular location:Translocates to lysosomal membranes in a calcium-dependent fashion.,
Specifications
| Size | 50μL/100μL |
| Target | cPLA2-ε |
| Reactivity | Human, Mouse |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | 96kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PLA2G4E. AA range:401-450 |
| Specificity | cPLA2-ε Polyclonal Antibody detects endogenous levels of cPLA2-ε protein. |
| Gene Name | PLA2G4E |
| Protein Name | Cytosolic phospholipase A2 epsilon |
| Other Name | PLA2G4E; / Cytosolic phospholipase A2 epsilon; / cPLA2-epsilon; / Phospholipase A2 group IVE |
| Species | Human |
| Gene ID | 123745 |
| Uniprot | Q3MJ16, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q50L42 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, cytosol . Early endosome membrane; Peripheral membrane protein; Cytoplasmic side . Lysosome membrane; Peripheral membrane protein; Cytoplasmic side . Cell membrane; Peripheral membrane protein; Cytoplasmic side . Targeted to clathrin-independent endocytotic vesicles through binding to phosphoinositides, especially phosphatidylinositol 4,5-bisphosphates. . |
| Tissue Expression | Heart,Lung,Tongue, |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Organismal Systems >> Nervous system >> Glutamatergic synapse;Organismal Systems >> Sensory system >> Inflammatory mediator regulation of TRP channels;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway |
| Research Areas | >>Glycerophospholipid metabolism; / >>Ether lipid metabolism; / >>Arachidonic acid metabolism; / >>Linoleic acid metabolism; / >>alpha-Linolenic acid metabolism; / >>Metabolic pathways; / >>MAPK signaling pathway; / >>Ras signaling pathway; / >>Phospholipase D signaling pathway; / >>Necroptosis; / >>Vascular smooth muscle contraction; / >>VEGF signaling pathway; / >>Platelet activation; / >>Fc epsilon RI signaling pathway; / >>Fc gamma R-mediated phagocytosis; / >>Glutamatergic synapse; / >>Serotonergic synapse; / >>Long-term depression; / >>Inflammatory mediator regulation of TRP channels; / >>GnRH signaling pathway; / >>Ovarian steroidogenesis; / >>Oxytocin signaling pathway; / >>Choline metabolism in cancer |
| Function | Catalytic activity:Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate.,Domain:The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).,enzyme regulation:Stimulated by cytosolic Ca(2+).,Function:Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position.,similarity:Contains 1 C2 domain.,similarity:Contains 1 PLA2c domain.,subcellular location:Translocates to lysosomal membranes in a calcium-dependent fashion., |
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