Crk II Polyclonal Antibody
Ucallm_WP11800-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | Crk II |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 40kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CrkII. AA range:187-236 |
| Specificity | Crk II Polyclonal Antibody detects endogenous levels of Crk II protein. |
| Gene Name | CRK |
| Protein Name | Adapter molecule crk |
| Other Name | CRK; / Adapter molecule crk; / Proto-oncogene c-Crk; / p38 |
| Species | Human |
| Gene ID | 1398 |
| Uniprot | P46108, |
| Species.1 | Mouse |
| Gene ID.1 | 12928 |
| Uniprot.1 | Q64010, |
| Species.2 | Rat |
| Gene ID.2 | 54245 |
| Uniprot.2 | Q63768 |
| Cellular Localization | Cytoplasm . Cell membrane . Translocated to the plasma membrane upon cell adhesion. . |
| Tissue Expression | Embryonic lung,Epithelium,Eye,Lung,Placenta, |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: specific types >> Chronic myeloid leukemia;Human Diseases >> Cancer: specific types >> Renal cell carcinoma;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway |
| Research Areas | >>MAPK signaling pathway; / >>ErbB signaling pathway; / >>Rap1 signaling pathway; / >>Chemokine signaling pathway; / >>Focal adhesion; / >>Fc gamma R-mediated phagocytosis; / >>Neurotrophin signaling pathway; / >>Regulation of actin cytoskeleton; / >>Insulin signaling pathway; / >>Growth hormone synthesis, secretion and action; / >>Bacterial invasion of epithelial cells; / >>Shigellosis; / >>Yersinia infection; / >>Human cytomegalovirus infection; / >>Human immunodeficiency virus 1 infection; / >>Pathways in cancer; / >>MicroRNAs in cancer; / >>Renal cell carcinoma; / >>Chronic myeloid leukemia |
| Function | Domain:The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.,Domain:The SH2 domain mediates interaction with SHB.,Function:The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.,PTM:Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.,PTM:Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 2 SH3 domains.,subcellular location:Translocated to the plasma membrane upon cell adhesion.,subunit:Interacts with ABL1, C3G, SOS, MAP4K1, MAPK8 and DOCK3 via its first SH3 domain. Interacts with BCAR1, CBL, CBLB, PXN, IRS4 and GAB1 via its SH2 domain upon stimulus-induced tyrosine phosphorylation. Interacts with several tyrosine-phosphorylated growth factor receptors such as EGFR, PDGFR and INSR via its SH2 domain (By similarity). Interacts with DOCK1 and DOCK4. Interacts with SHB., |
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