Price range: $268.00 through $328.00
Background:
This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009],
| Target | Crk-L |
| Reactivity | Human |
| Applications | WB, FC, ELISA |
| MW(Calculated) | 34kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | WB 1:500-1:2000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | |
| Purification | Affinity purification |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 11H8 |
| Immunogen | Purified recombinant fragment of human Crk-L expressed in E. Coli. |
| Sequence | |
| Specificity | Crk-L Monoclonal Antibody detects endogenous levels of Crk-L protein. |
| Gene Name | CRKL |
| Protein Name | Crk-like protein |
| Other Name | CRKL; / Crk-like protein |
| Species | Human |
| Gene ID-1 | 1399 |
| Uniprot | P46109, |
| Species.1 | Mouse |
| Gene ID-2 | 12929 |
| Uniprot.1 | P47941 |
| Species.2 | |
| Gene ID-3 | |
| Uniprot.2 | |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009], |
| Cellular Localization | endosome,cytosol,cell-cell adherens junction,extracellular exosome, |
| Tissue Expression | Skin,Spleen, |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: specific types >> Chronic myeloid leukemia;Human Diseases >> Cancer: specific types >> Renal cell carcinoma;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway |
| Research Areas | >>MAPK signaling pathway; / >>ErbB signaling pathway; / >>Rap1 signaling pathway; / >>Chemokine signaling pathway; / >>Focal adhesion; / >>Fc gamma R-mediated phagocytosis; / >>Neurotrophin signaling pathway; / >>Regulation of actin cytoskeleton; / >>Insulin signaling pathway; / >>Growth hormone synthesis, secretion and action; / >>Bacterial invasion of epithelial cells; / >>Shigellosis; / >>Yersinia infection; / >>Human cytomegalovirus infection; / >>Human immunodeficiency virus 1 infection; / >>Pathways in cancer; / >>MicroRNAs in cancer; / >>Renal cell carcinoma; / >>Chronic myeloid leukemia |
| Function | Function:May mediate the transduction of intracellular signals.,similarity:Contains 1 SH2 domain.,similarity:Contains 2 SH3 domains.,subunit:Interacts with INPP5D/SHIP1. Interacts with DOCK2 and EPOR. Interacts with phosphorylated CBLB and IRS4., |
| RRID |
