Name: CrkII mouse mAb
SKU: WM0661
Price: 268 USD
Availability: InStock

Product details

Background:
This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	Crk II
Reactivity	Human
Applications	WB
MW(Calculated)
MW(Observed)	34kD
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:1000
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	5B10
Immunogen	Purified recombinant human CrkII protein fragments expressed in E.coli.
Specificity	This antibody detects endogenous levels of CrkII and does not cross-react with related proteins.
Gene Name	crk
Protein Name
Other Name	Adapter molecule crk; / Avian sarcoma virus CT10; / v crk; / oncogene homolog; / CRK; / CRK isoform 2; / CRK isoform II; / CRK_HUMAN; / CRKII; / FLJ38130; / OTTHUMP00000115366; / OTTHUMP00000198330; / p38; / Proto oncogene C crk; / Proto-oncogene C-crk; / v crk avian sarcoma virus CT10 oncogene homolog; / v crk sarcoma virus CT10 oncogene homolog; / v crk sarcoma virus CT10 oncogene homolog; / avian; / .
Species	Human
Gene ID	1398
Uniprot	P46108,
Species.1	Mouse
Gene ID.1	12928
Uniprot.1	Q64010
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasm . Cell membrane . Translocated to the plasma membrane upon cell adhesion. .
Tissue Expression	Embryonic lung,Epithelium,Eye,Lung,Placenta,
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: specific types >> Chronic myeloid leukemia;Human Diseases >> Cancer: specific types >> Renal cell carcinoma;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway
Research Areas	>>MAPK signaling pathway; / >>ErbB signaling pathway; / >>Rap1 signaling pathway; / >>Chemokine signaling pathway; / >>Focal adhesion; / >>Fc gamma R-mediated phagocytosis; / >>Neurotrophin signaling pathway; / >>Regulation of actin cytoskeleton; / >>Insulin signaling pathway; / >>Growth hormone synthesis, secretion and action; / >>Bacterial invasion of epithelial cells; / >>Shigellosis; / >>Yersinia infection; / >>Human cytomegalovirus infection; / >>Human immunodeficiency virus 1 infection; / >>Pathways in cancer; / >>MicroRNAs in cancer; / >>Renal cell carcinoma; / >>Chronic myeloid leukemia
Function	Domain:The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.,Domain:The SH2 domain mediates interaction with SHB.,Function:The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.,PTM:Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.,PTM:Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 2 SH3 domains.,subcellular location:Translocated to the plasma membrane upon cell adhesion.,subunit:Interacts with ABL1, C3G, SOS, MAP4K1, MAPK8 and DOCK3 via its first SH3 domain. Interacts with BCAR1, CBL, CBLB, PXN, IRS4 and GAB1 via its SH2 domain upon stimulus-induced tyrosine phosphorylation. Interacts with several tyrosine-phosphorylated growth factor receptors such as EGFR, PDGFR and INSR via its SH2 domain (By similarity). Interacts with DOCK1 and DOCK4. Interacts with SHB.,