CtIP Polyclonal Antibody
Ucallm_WP11831-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | CtIP |
| Reactivity | Human, Mouse |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | 102kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RBBP8. AA range:630-679 |
| Specificity | CtIP Polyclonal Antibody detects endogenous levels of CtIP protein. |
| Gene Name | RBBP8 |
| Protein Name | DNA endonuclease RBBP8 |
| Other Name | RBBP8; / CTIP; / DNA endonuclease RBBP8; / CtBP-interacting protein; / CtIP; / Retinoblastoma-binding protein 8; / RBBP-8; / Retinoblastoma-interacting protein and myosin-like; / RIM; / Sporulation in the absence of SPO11 protein 2 homolog; / SAE2 |
| Species | Human |
| Gene ID | 5932 |
| Uniprot | Q99708, |
| Species.1 | Mouse |
| Gene ID.1 | 225182 |
| Uniprot.1 | Q80YR6 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Chromosome . Associates with sites of DNA damage in S/G2 phase (PubMed:10764811, PubMed:25349192). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604). . |
| Tissue Expression | Expressed in ER-positive breast cancer lines, but tends to be down-regulated ER-negative cells (at protein level). |
| Signaling_pathway | |
| Research Areas | >>Homologous recombination |
| Function | Function:May modulate the functions ascribed to BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1.,PTM:Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.,subcellular location:Predominantly nuclear.,subunit:Interacts with CTBP, with the C-terminal (BRCT) domains of BRCA1, and with the retinoblastoma protein., |
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