Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | Cyclin B1 |
| Reactivity | Human, Rat |
| Applications | WB, IP, IF, FC, ELISA |
| MW(Calculated) | 48kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 1G9 |
| Immunogen | Purified recombinant fragment of human Cyclin B1 expressed in E. Coli. |
| Specificity | Cyclin B1 Monoclonal Antibody detects endogenous levels of Cyclin B1 protein. |
| Gene Name | CCNB1 CCNB |
| Protein Name | G2/mitotic-specific cyclin-B1 |
| Other Name | CCNB1; / CCNB; / G2/mitotic-specific cyclin-B1 |
| Species | Human |
| Gene ID | 891 |
| Uniprot | P14635, |
| Species.1 | Mouse |
| Gene ID.1 | 268697 |
| Uniprot.1 | P24860, |
| Species.2 | Rat |
| Gene ID.2 | 25203 |
| Uniprot.2 | P30277 |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. |
| Tissue Expression | Breast adenocarcinoma,Lung,Placenta, |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Cell cycle;Cellular Processes >> Cell growth and death >> p53 signaling pathway;Cellular Processes >> Cell growth and death >> Cellular senescence;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway |
| Research Areas | >>FoxO signaling pathway; / >>Cell cycle; / >>Oocyte meiosis; / >>p53 signaling pathway; / >>Cellular senescence; / >>Progesterone-mediated oocyte maturation; / >>Human immunodeficiency virus 1 infection |
| Function | developmental stage:Accumulates steadily during G2 and is abruptly destroyed at mitosis.,Function:Essential for the control of the cell cycle at the G2/M (mitosis) transition.,PTM:Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.,similarity:Belongs to the cyclin family.,similarity:Belongs to the cyclin family. Cyclin AB subfamily.,subunit:Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase., |
