SKU: WP11897 Categories: ,

CYP39A1 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP11897-100
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Product details

Background:

cytochrome P450 family 39 subfamily A member 1(CYP39A1) Homo sapiens This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is involved in the conversion of cholesterol to bile acids. Its substrates include the oxysterols 25-hydroxycholesterol, 27-hydroxycholesterol and 24-hydroxycholesterol. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],

Specifications

Size50μL/100μL
TargetCYP39A1
ReactivityHuman, Mouse
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)55kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenSynthesized peptide derived from the Internal region of human CYP39A1.
SpecificityCYP39A1 Polyclonal Antibody detects endogenous levels of CYP39A1 protein.
Gene NameCYP39A1
Protein Name24-hydroxycholesterol 7-alpha-hydroxylase
Other NameCYP39A1; / 24-hydroxycholesterol 7-alpha-hydroxylase; / Cytochrome P450 39A1; / hCYP39A1; / Oxysterol 7-alpha-hydroxylase
SpeciesHuman
Gene ID51302
UniprotQ9NYL5,
Species.1Mouse
Gene ID.156050
Uniprot.1Q9JKJ9
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationEndoplasmic reticulum membrane; Multi-pass membrane protein . Microsome membrane; Multi-pass membrane protein .
Tissue ExpressionLiver specific.
Signaling_pathway
Research Areas>>Primary bile acid biosynthesis
FunctionCatalytic activity:(24R)-cholest-5-ene-3-beta,24-diol + NADPH + O(2) = (24R)-cholest-5-ene-3-beta,7-alpha,24-triol + NADP(+) + H(2)O.,cofactor:Heme group.,Function:Involved in the bile acid metabolism. Has a preference for 24-hydroxycholesterol, and converts it into a 7-alpha-hydroxylated product.,similarity:Belongs to the cytochrome P450 family.,tissue specificity:Liver specific.,
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