Name: Cytokeratin 10 [PTR1365/056] mouse mAb
SKU: WM1381
Price: 268 USD
Availability: InStock

Product details

Background:
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	Cytokeratin 10
Reactivity	Human
Applications	IHC, WB, IF, ELISA
MW(Calculated)	60kD
MW(Observed)	70kD
Host Species	Mouse
Isotype	IgG1,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RMMR1365/056
Immunogen	Synthesized peptide derived from human protein.AA range: 100-200
Specificity	This antibody detects endogenous levels of Cytokeratin 10.
Gene Name	KRT10 KPP
Protein Name	Cytokeratin-10
Other Name	Keratin, type I cytoskeletal 10; / Cytokeratin-10; / CK-10; / Keratin-10; / K10
Species	Human
Gene ID	3858
Uniprot	P13645
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Membranous, Cytoplasmic
Tissue Expression	Seen in all suprabasal cell layers including stratum corneum. Expressed on the surface of lung cell lines (PubMed:19627498).
Signaling_pathway	Organismal Systems >> Endocrine system >> Estrogen signaling pathway
Research Areas	>>Estrogen signaling pathway; / >>Staphylococcus aureus infection
Function	Disease:Defects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.,Disease:Defects in KRT10 are a cause of epidermal nevus epidermolytic hyperkeratotic type [MIM:600648]. Epidermal nevi affect about 1 in 1,000 people. They appear at or shortly after birth as localized lines of epidermal thickening. The extent of skin involvement varies widely.,Disease:Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,online information:Keratin-10 entry,polymorphism:A number of alleles are known that mainly differ in the Gly-rich region (positions 490-560).,similarity:Belongs to the intermediate filament family.,subunit:Heterotetramer of two type I and two type II keratins. keratin-10 is generally associated with keratin-1.,tissue specificity:Seen in all suprabasal cell layers including stratum corneum.,