Name: Cytokeratin 8 [ABT535] mouse mAb
SKU: WM1409
Price: 268 USD
Availability: InStock

Cytokeratin 8 [ABT535] mouse mAb

Ucallm_WM1409-50

Price range: $268.00 through $328.00

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50μl

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SKU: WM1409-50

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Product details

Background:
Cytokeratin 8 (CK8) is a member of the type II family of cytokeratins with a molecular weight of 53 kDa which plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. It generally exists in monolayer and pseudostratified epithelium, and is expressed in glandular epithelium, transitional epithelium and hepatocytes. It is absent in squamous cell carcinomas. CK8 dimerizes with CK18 to form an intermediate filament in simple single-layered epithelial cells. CK8/18 expression patterns are useful for classification of tumors of unknown origin and poorly differentiated carcinomas.

Specifications

Size	50μL/100μL
Target	Cytokeratin 8
Reactivity	Human, Mouse, Rat
Applications	IHC, WB, IF, ELISA
MW(Calculated)	54kD
MW(Observed)	54kD
Host Species	Mouse
Isotype	IgG2a,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RMM535
Immunogen	Synthesized peptide derived from human Cytokeratin 8 AA range: 400-483
Specificity	The antibody can specifically recognize human CK8 protein, and shows no cross reaction with CK1, 4, 5, 6, 7, 10, 15, 16, 17, 18, 20.
Gene Name	KRT8 CYK8
Protein Name	CARD2; CK 8; CK-8; CK8; CYK8; CYKER; Cytokeratin endo A; Cytokeratin-8; DreK8; EndoA; K2C8; K2C8_HUMAN; K8; Keratin 8; Keratin type II cytoskeletal 8; Keratin, type II cytoskeletal 8; Keratin-8; KO; Krt 2.8; KRT8; MGC118110; MGC174782; MGC53564; MGC85764; sb:cb186; Type-II keratin Kb8
Other Name	CARD2; / CK 8; / CK-8; / CK8; / CYK8; / CYKER; / Cytokeratin endo A; / CytokeRatin-8; / DreK8; / EndoA; / K2C8; / K2C8_HUMAN; / K8; / KeRatin 8; / KeRatin type II cytoskeletal 8; / KeRatin, type II cytoskeletal 8; / KeRatin-8; / KO; / Krt 2.8; / KRT8; / MGC118110; / MGC174782; / MGC53564; / MGC85764; / sb:cb186; / Type-II keRatin Kb8
Species	Human
Gene ID	3856
Uniprot	P05787,
Species.1	Mouse
Gene ID.1	16691
Uniprot.1	P11679,
Species.2	Rat
Gene ID.2	25626
Uniprot.2	Q10758
Cellular Localization	Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix .
Tissue Expression	Liver/ Tonsil
Signaling_pathway
Research Areas
Function	Disease:Defects in KRT8 are a cause of cryptogenic cirrhosis [MIM:215600].,Function:Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,PTM:O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.,PTM:Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.,similarity:Belongs to the intermediate filament family.,subunit:Heterotetramer of two type I and two type II keratins. keratin-8 associates with keratin-18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP.,tissue specificity:Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.,