Price range: $268.00 through $328.00
Background:
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],
| Size | 50μL/100μL |
| Target | DARPP-32 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 32kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DARPP-32. AA range:41-90 |
| Specificity | DARPP-32 Polyclonal Antibody detects endogenous levels of DARPP-32 protein. |
| Gene Name | PPP1R1B |
| Protein Name | Protein phosphatase 1 regulatory subunit 1B |
| Other Name | PPP1R1B; / DARPP32; / Protein phosphatase 1 regulatory subunit 1B; / DARPP-32; / Dopamine- and cAMP-regulated neuronal phosphoprotein |
| Species | Human |
| Gene ID | 84152 |
| Uniprot | Q9UD71, |
| Species.1 | Mouse |
| Gene ID.1 | 19049 |
| Uniprot.1 | Q60829, |
| Species.2 | Rat |
| Gene ID.2 | 360616 |
| Uniprot.2 | Q6J4I0 |
| Cellular Localization | Cytoplasm. |
| Tissue Expression | Adipose tissue,Brain,Cerebellum,Colon,Ovary, |
| Signaling_pathway | Organismal Systems >> Nervous system >> Dopaminergic synapse;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway |
| Research Areas | >>cAMP signaling pathway; / >>Dopaminergic synapse; / >>Cocaine addiction; / >>Amphetamine addiction; / >>Alcoholism |
| Function | Function:Inhibitor of protein-phosphatase 1.,PTM:Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.,PTM:Phosphorylation of Thr-34 is required for activity.,similarity:Belongs to the protein phosphatase inhibitor 1 family., |
