Name: U-Blot® DDR1 Rabbit mAb
SKU: WR4687
Price: 268 USD
Availability: InStock

Product details

Background:Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011],

Specifications

Size	50μL/100μL
Target	DDR1
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	101kD
MW(Observed)	125kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0981R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	DDR1
Protein Name	Epithelial discoidin domain-containing receptor 1
Other Name	DDR1; / CAK; / EDDR1; / NEP; / NTRK4; / PTK3A; / RTK6; / TRKE; / Epithelial discoidin domain-containing receptor 1; / Epithelial discoidin domain receptor 1; / CD167 antigen-like family member A; / Cell adhesion kinase; / Discoidin receptor tyrosine kinase; / HGK2
Species	Human
Gene ID	780
Uniprot	Q08345,
Species.1	Mouse
Gene ID.1	12305
Uniprot.1	Q03146,
Species.2	Rat
Gene ID.2
Uniprot.2	Q63474
Cellular Localization	[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted .; [Isoform 4]: Cell membrane; Single-pass type I membrane protein.
Tissue Expression	Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.
Signaling_pathway
Research Areas
Function	Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Domain:The Gly/Pro-rich domains may be required for an unusual geometry of interaction with ligand or substrates.,Function:May be involved in cell-cell interactions and recognition.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.,similarity:Contains 1 F5/8 type C domain.,similarity:Contains 1 protein kinase domain.,tissue specificity:Expressed at low levels in most adult tissues and is highest in the brain and lung. Abundant in breast carcinoma cell lines.,