SKU: WM0142 Categories: ,

DDX4 Monoclonal Antibody

Price range: $268.00 through $328.00

SKU: WM0142-50
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Product details

Background:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009],

Specifications

Size50μL/100μL
TargetDDX4
ReactivityHuman
ApplicationsWB, IHC, IF, FC, ELISA
MW(Calculated)79kD
MW(Observed)
Host SpeciesMouse
Isotype
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationAffinity purification
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone Number12D4
ImmunogenPurified recombinant fragment of human DDX4 expressed in E. Coli
SpecificityDDX4 Monoclonal Antibody detects endogenous levels of DDX4 protein.
Gene NameDDX4
Protein NameProbable ATP-dependent RNA helicase DDX4
Other NameDDX4; / VASA; / Probable ATP-dependent RNA helicase DDX4; / DEAD box protein 4; / Vasa homolog
SpeciesHuman
Gene ID54514
UniprotQ9NQI0,
Species.1Mouse
Gene ID.1
Uniprot.1Q61496
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationCytoplasm . Cytoplasm, perinuclear region . Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. .
Tissue ExpressionExpressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.
Signaling_pathway
Research Areas
FunctionFunction:May play a role in germ cell development.,similarity:Belongs to the DEAD box helicase family.,similarity:Belongs to the DEAD box helicase family. DDX4/VASA subfamily.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,subunit:N-terminus interacts with RANBP9. Interacts with PIWIL2 and MAEL.,tissue specificity:Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.,
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