DGK-α Polyclonal Antibody
Ucallm_WP11993-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | DGK-α |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 80kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DGKA. AA range:304-353 |
| Specificity | DGK-α Polyclonal Antibody detects endogenous levels of DGK-α protein. |
| Gene Name | DGKA |
| Protein Name | Diacylglycerol kinase alpha |
| Other Name | DGKA; / DAGK; / DAGK1; / Diacylglycerol kinase alpha; / DAG kinase alpha; / 80 kDa diacylglycerol kinase; / Diglyceride kinase alpha; / DGK-alpha |
| Species | Human |
| Gene ID | 1606 |
| Uniprot | P23743, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | O88673 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, cytosol . |
| Tissue Expression | Expressed in lymphocytes. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway |
| Research Areas | >>Glycerolipid metabolism; / >>Glycerophospholipid metabolism; / >>Metabolic pathways; / >>Phosphatidylinositol signaling system; / >>Phospholipase D signaling pathway; / >>Choline metabolism in cancer |
| Function | Catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,enzyme regulation:Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C.,Function:Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 2 EF-hand domains.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Monomer.,tissue specificity:Lymphocytes and oligodendroglial cells., |
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