Dynactin 2 Polyclonal Antibody
Ucallm_WP12080-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012],
Specifications
| Size | 50μL/100μL |
| Target | Dynactin 2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 50kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p50 Dynamitin. AA range:341-390 |
| Specificity | Dynactin 2 Polyclonal Antibody detects endogenous levels of Dynactin 2 protein. |
| Gene Name | DCTN2 |
| Protein Name | Dynactin subunit 2 |
| Other Name | DCTN2; / DCTN50; / Dynactin subunit 2; / 50 kDa dynein-associated polypeptide; / Dynactin complex 50 kDa subunit; / DCTN-50; / p50 dynamitin |
| Species | Human |
| Gene ID | 10540 |
| Uniprot | Q13561, |
| Species.1 | Mouse |
| Gene ID.1 | 69654 |
| Uniprot.1 | Q99KJ8, |
| Species.2 | Rat |
| Gene ID.2 | 299850 |
| Uniprot.2 | Q6AYH5 |
| Cellular Localization | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Membrane; Peripheral membrane protein . |
| Tissue Expression | Brain,Embryonic kidney,Fetal brain cortex,Placenta,Platelet,Skin,Uterus, |
| Signaling_pathway | Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases |
| Research Areas | >>Vasopressin-regulated water reabsorption; / >>Amyotrophic lateral sclerosis; / >>Huntington disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Salmonella infection |
| Function | Function:Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. May play a role in synapse formation during brain development.,similarity:Belongs to the dynactin subunit 2 family.,subunit:Subunit of dynactin, a multiprotein complex associated with dynein. Interacts with BICD2 (By similarity). Interacts with MAPRE1., |
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