Price range: $268.00 through $328.00
Background:
This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | eEF2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| MW(Calculated) | 95kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000-1:2000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 9E1 |
| Immunogen | Purified recombinant human EF-2 protein fragments expressed in E.coli. |
| Specificity | EF-2 Monoclonal Antibody detects endogenous levels of EF-2 protein. |
| Gene Name | EEF2 |
| Protein Name | Elongation factor 2 |
| Other Name | EEF2; / EF2; / Elongation factor 2; / EF-2 |
| Species | Human |
| Gene ID | 1938 |
| Uniprot | P13639, |
| Species.1 | Mouse |
| Gene ID.1 | 13629 |
| Uniprot.1 | P58252, |
| Species.2 | Rat |
| Gene ID.2 | 29565 |
| Uniprot.2 | P05197 |
| Cellular Localization | Cytoplasm . Nucleus . Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product. . |
| Tissue Expression | Brain,Cajal-Retzius cell,Epithelium,Hepatocyte,Ovary,Periph |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway |
| Research Areas | >>AMPK signaling pathway; / >>Oxytocin signaling pathway |
| Function | Function:This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome.,PTM:Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.,PTM:Phosphorylation by EF-2 kinase completely inactivates EF-2.,similarity:Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.,subunit:Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9., |
