SKU: WP9123 Categories: ,

EGFR (phospho Tyr1172) Polyclonal Antibody

Price range: $288.00 through $368.00

SKU: WP9123-100
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Product details

Background:

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016],

Specifications

TargetEGFR
ReactivityHuman, Mouse, Rat
ApplicationsWB, ELISA
MW(Calculated)
MW(Observed)175kD
Host SpeciesRabbit
IsotypeIgG
Conjugate
ModificationPhospho
Recommended Dilution RatioWB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
CompositionLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification ProcessThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage Condition-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Tyr1172. AA range:1139-1188
Sequence
SpecificityPhospho-EGFR (Y1172) Polyclonal Antibody detects endogenous levels of EGFR protein only when phosphorylated at Y1172.
Gene NameEGFR
Protein NameEpidermal growth factor receptor
AliasEGFR;ERBB;ERBB1;HER1;Epidermal growth factor receptor;Proto-oncogene c-ErbB-1;Receptor tyrosine-protein kinase erbB-1
Organism-1Human
Gene ID-11956
SwissProt-1P00533
Organism-2Mouse
Gene ID-213649
SwissProt-2Q01279
Organism-3
Gene ID-3
SwissProt-3
Organism-4
Gene ID-4
SwissProt-4
Cellular LocalizationCell membrane ; Single-pass type I membrane protein . Endoplasmic reticulum membrane ; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome . Endosome membrane. Nucleus . In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER (PubMed:20674546, PubMed:17909029). Endocytosed upon activation by ligand (PubMed:2790960, PubMed:17182860, PubMed:27153536, PubMed:17909029). Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF) (PubMed:20551055). .; [Isoform 2]: Secreted.
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