eIF4E Monoclonal Antibody
Ucallm_WM0154-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015],
Specifications
| Size | 50μL/100μL |
| Target | eIF4E |
| Reactivity | Human |
| Applications | WB, IHC, IF, FC, ELISA |
| MW(Calculated) | 25kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 8D4 |
| Immunogen | Purified recombinant fragment of human eIF4E expressed in E. Coli. |
| Specificity | eIF4E Monoclonal Antibody detects endogenous levels of eIF4E protein. |
| Gene Name | EIF4E |
| Protein Name | Eukaryotic translation initiation factor 4E |
| Other Name | EIF4E; / EIF4EL1; / EIF4F; / Eukaryotic translation initiation factor 4E; / eIF-4E; / eIF4E; / eIF-4F 25 kDa subunit; / mRNA cap-binding protein |
| Species | Human |
| Gene ID | 1977 |
| Uniprot | P06730, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P63073 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, P-body . Cytoplasm . Cytoplasm, Stress granule . Nucleus . Interaction with EIF4ENIF1/4E-T is required for localization to processing bodies (P-bodies) (PubMed:16157702, PubMed:24335285, PubMed:25923732). Imported in the nucleus via interaction with EIF4ENIF1/4E-T via a piggy-back mechanism (PubMed:10856257). . |
| Tissue Expression | Brain,Fetal brain,Placenta,Pooled,Small intestine,Testis, |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway |
| Research Areas | >>EGFR tyrosine kinase inhibitor resistance; / >>HIF-1 signaling pathway; / >>mTOR signaling pathway; / >>PI3K-Akt signaling pathway; / >>Longevity regulating pathway; / >>Insulin signaling pathway |
| Function | Caution:Was originally thought to be phosphorylated on Ser-53 (PubMed:3112145); this was later shown to be wrong (PubMed:7665584).,Function:Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.,PTM:Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.,similarity:Belongs to the eukaryotic initiation factor 4E family.,subunit:eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. Interacts mutually exclusive with EIF4A1 and EIF4A2. Interacts with NGDN and PIWIL2 (By similarity). Interacts with Lassa virus Z protein., |
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