Price range: $268.00 through $328.00
Background:
Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM, May 2010],
| Size | 50μL/100μL |
| Target | eIF5 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | 49kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from the Internal region of human eIF5. |
| Specificity | eIF5 Polyclonal Antibody detects endogenous levels of eIF5 protein. |
| Gene Name | EIF5 |
| Protein Name | Eukaryotic translation initiation factor 5 |
| Other Name | EIF5; / Eukaryotic translation initiation factor 5; / eIF-5 |
| Species | Human |
| Gene ID | 1983 |
| Uniprot | P55010, |
| Species.1 | Mouse |
| Gene ID.1 | 217869 |
| Uniprot.1 | P59325, |
| Species.2 | Rat |
| Gene ID.2 | 56783 |
| Uniprot.2 | Q07205 |
| Cellular Localization | nucleus,cytoplasm,cytosol,plasma membrane,cell-cell adherens junction, |
| Tissue Expression | Brain,Epithelium,Lymph,Pooled,Uterus, |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).,similarity:Belongs to the eIF-2-beta/eIF-5 family.,similarity:Contains 1 W2 domain., |
