eIF5B Polyclonal Antibody
Ucallm_WP12153-50
Price range: $268.00 through $328.00
Product details
Background:
Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | eIF5B |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 139kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EIF5B. AA range:1051-1100 |
| Specificity | eIF5B Polyclonal Antibody detects endogenous levels of eIF5B protein. |
| Gene Name | EIF5B |
| Protein Name | Eukaryotic translation initiation factor 5B |
| Other Name | EIF5B; / IF2; / KIAA0741; / Eukaryotic translation initiation factor 5B; / eIF-5B; / Translation initiation factor IF-2 |
| Species | Human |
| Gene ID | 9669 |
| Uniprot | O60841, |
| Species.1 | Mouse |
| Gene ID.1 | 226982 |
| Uniprot.1 | Q05D44, |
| Species.2 | Rat |
| Gene ID.2 | 308306 |
| Uniprot.2 | B2GUV7 |
| Cellular Localization | Cytoplasm . |
| Tissue Expression | Brain,Cervix carcinoma,Epithelium,Hepatocyte,Liver,Lymph,Pl |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.,similarity:Belongs to the IF-2 family.,subunit:Interacts with ANXA5 in a calcium and phospholipid-dependent manner., |
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